KMID : 0895820110210010047
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Journal of Oriental Rehabilitation Medicine 2011 Volume.21 No. 1 p.47 ~ p.56
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The Biological Activities of Extracts and Fractions of Herbal Plants
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Kim Jeong-Hwan
Ko Youn-Seok
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Abstract
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Objectives: This study was conducted to identify the antimicrobial anti-oxidative and tyrosinase inhibitory effect of three kinds of herbal plants, Polygonum cuspidatum, Chelidonii herba, Pharbitis nil L. naturally grown across the nation.
Methods: To investigate in vitro anti-oxidative activity assay, antimicrobial effect and tyrosinase inhibitory activity, MeOH 80% extract, n-hexane, chloroform, ethyl acetate, n-butanol and water fractions of herbal plants were tested by diphenylpicrylhydrazyl(DPPH) method.
Results: Bsutanol fractions of each plants showed about 90% on anti-oxidative effect. In case of tyrosinase activity, ethlyacetate fraction of Polygonum cuspidatum showed a potent inhibition effect. In the antimicrobial effect, ethylacetate fraction of Polygonum cuspidatu, hexane fractions of Chelidonii herba and the methanol 80% extracts of Pharbitis nil L. exhibited inhibition effect significantly.
Conclusions :In MeOH 80% extracts, we couldn`t found any extracts or fractions which have antimicrobial, anti-oxidative and tyrosinase inhibitory activity, however ethylacetate fractions of Polygonum cuspidatum showed effects commonly in these three assay system.
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KEYWORD
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Biological activities, Polygonum cuspidatum, Chelidonii herba, Pharbitis nil L., Anti-oxidative
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